Development of fluorescent probes for visualizing the pH-based singularity inside bodies
Development of the non-invasive method for detection of transformed cells in human stem cell cultures
Development of polymer caged luciferin enabling spatiotemporal trans-scale imaging
Finding and Understanding Singularity Phenomena Using 4D Photoacoustic Imaging
|Dept. of Medical Engineering, National Defense Medical College
|Biomedical optical engineering
|Photoacoustic (PA) imaging based on optical absorption and ultrasonic detection has the advantage of imaging that can penetrate deeply without compromising on visualization depth or imaging resolution. This study targets the spatiotemporal dynamics of singularity cell using PA imaging technology. We will establish cross-organ 4D PA spectroscopic imaging in order to find singularity cell as discontinuous change.
Mechanistic Modeling of Crowds Led by Cells of Singularity
Development of single cell multi-omics tecnique for identification of singularity cells
Detection of singularity neurons during neural network formation
Identifying singular cells based on quantification of unpredictabiliy through spatio-temporal modeling
Singularity in influenza virus infection
Cancer-promoting singularity cells in pancreatic cancer
Elucidation of the principle of ploidy reversal in tumorigenesis
|Hokkaido University, Faculty of Advanced Life Science
|Whole genome duplication and subsequent chromosome loss (ploidy reversal), which drastically change cellular phenotypes, are hallmarks of cancer. However, as ploidy reversal happens at extremely low frequency both in cell cultures and tissues, it is difficult to specify cellular conditions that evoke the event and to trace its long-term consequences in the process of tumorigenesis. In this study, using a new imaging system that enables simultaneous high spatiotemporal imaging and long-term cell lineage tracing, we will identify rare cellular states that cause ploidy reversal and elucidate its principle and pathological contribution in tumorigenesis.
Imaging of synapse elimination in the developing brain
Singularity in the collectives state transition in Dictyostelium movement
Life and death decisions on encephalitis
Mathematical modeling of outlier immune cells using extreme statistics
Identification of singularity cells that masculinize a primary organizer in response to environmental stimuli
Elucidation of singularity events during immune reactions by Time-dependent Immunology and single cell analysis
Identification and functional analysis of singularity cells on intratumoral heterogeneity
Analysis of the singularity phenomenon by mosaicism using disease-specific iPS cells
|Kitasato University School of Allied Health Sciences
|Tau is related to cognitive dysfunction that is one of non-motor symptoms of Parkinson’s disease (PD). In this study, to elucidate the propagation of oligomer-Tau and its degradation mechanism by singularity phenomenon, we analyze disease-specific iPS cells (iPSC) from familial PD patient with LRRK2 mutation. Furthermore, to investigate whether singularity cells with oligomer-Tau are caused by mosaicism of mutant LRRK2 allele, we analyze iPSC-derived neurons under in vitro and in vivo conditions.
Singularity determining development of autoimmune diseases
Evocation of singularity at emergence of cancer cells in normal stroma
|Tokyo University of Science, Research Institute for Biomedical Sciences, Division of Development and Aging
|Tumor microenvironment (TME) is a specialized, complex compartment comprising of tumor-educated stromal cells, which promotes both survival and proliferation of cancer cells. However, it remains enigmatic what happens at emergence of cancer cells in stroma, where normal stromal cells face to cancer cells at the first time. Our preliminary results suggest that normal stroma countervails the expansion of low-grade cancer cells, while high-grade cancer cells are able to transform normal storma into TME. In this study, we aim to reveal the nature of singularity at the initial stage of TME formation by combined ex vivo imaging and omics approaches.